LOCUS       40924_23609       12254 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB661 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12254)
  AUTHORS   Nakamura S, Mano S, Tanaka Y, Ohnishi M, Nakamori C, Araki M, Niwa 
            T, Nishimura M, Kaminaka H, Nakagawa T, Sato Y, Ishiguro S.
  TITLE     Gateway binary vectors with the bialaphos resistance gene, bar, as a
            selection marker for plant transformation
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (6), 1315-1319 (2010)
  PUBMED    20530878
REFERENCE   2  (bases 1 to 12254)
  AUTHORS   Tanaka Y, Nakamura S, Ishiguro S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12254)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12254)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "6"; 
            pages: "1315-1319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1888
                     /codon_start=1
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     protein_bind    1898..2022
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2059..2089
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2143..2799
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3144..3446
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3490..3614)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3640..3892
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3926..3942)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3950..3966
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3974..4004)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4019..4040)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4076..4328)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4491..5039)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5091..5270)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5776..5800)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6321..7109
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7358..7946
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8132..8272)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8616..8810)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8879..9949)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10381..11007)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg gtgtctaagg gcgaagagct
     1201 gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
     1261 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     1321 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
     1381 cggcagcaga accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt
     1441 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     1501 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     1561 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaacac
     1621 cgagatgctg taccccgctg acggcggcct ggaaggcaga agcgacatgg ccctgaagct
     1681 cgtgggcggg ggccacctga tctgcaactt caagaccaca tacagatcca agaaacccgc
     1741 taagaacctc aagatgcccg gcgtctacta tgtggaccac agactggaaa gaatcaagga
     1801 ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     1861 ccctagcaaa ctggggcaca aacttaatgc tgttatcaca agtttgtaca aaaaagctga
     1921 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
     1981 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
     2041 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
     2101 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     2161 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     2221 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     2281 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     2341 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     2401 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     2461 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     2521 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     2581 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     2641 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     2701 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     2761 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta
     2821 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     2881 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     2941 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     3001 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     3061 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     3121 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     3181 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     3241 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     3301 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     3361 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     3421 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     3481 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     3541 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     3601 ttgtacaaag tggtgataac ctagagctcg aatttccccg atcgttcaaa catttggcaa
     3661 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     3721 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     3781 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     3841 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     3901 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3961 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4021 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctattaa ttcccgatct
     4081 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
     4141 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
     4201 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
     4261 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
     4321 gaacgatcgg ggaaattcga gctggggacg tccccagctc ggtaccccct cctgggcacg
     4381 gtcgtacgcg atccgccgcg gttccacctc accattgcgg gtatgccagt tgagttcgat
     4441 gagttcccgt accagctcca actcgggcga tgccacgcgc actccgttca gatctcggtg
     4501 acgggcagga ccggacgggg cggtaccggc aggctgaagt ccagctgcca gaaacccacg
     4561 tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg
     4621 agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc
     4681 ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc
     4741 gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg
     4801 cgtgccttcc aggggcccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg
     4861 agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc
     4921 ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc
     4981 gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggctcata
     5041 tcttattgcc ccccgggatc agatctggat tgagagtgaa tatgagactc taattggata
     5101 ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga
     5161 tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc
     5221 attaaactcc agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc
     5281 aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc
     5341 tccgctcatg atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
     5401 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg
     5461 cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc
     5521 tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc
     5581 cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc
     5641 ttgcgccctg agtgcttgcg gcagcgtgaa gctgatccgt cgagattaat agaaataaat
     5701 tcagcctaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
     5761 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     5821 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     5881 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     5941 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     6001 tgattgtaac gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga
     6061 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     6121 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     6181 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     6241 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     6301 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     6361 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     6421 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     6481 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     6541 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     6601 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     6661 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     6721 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     6781 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     6841 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     6901 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     6961 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     7021 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     7081 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     7141 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     7201 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac
     7261 acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact
     7321 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7381 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7441 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7501 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7561 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7621 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7681 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7741 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7801 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7861 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7921 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7981 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8041 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8101 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     8161 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     8221 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     8281 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     8341 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     8401 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt
     8461 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg
     8521 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt
     8581 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa
     8641 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat
     8701 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt
     8761 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac
     8821 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg
     8881 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag
     8941 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc
     9001 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct
     9061 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac
     9121 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct
     9181 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc
     9241 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg
     9301 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta
     9361 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga
     9421 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt
     9481 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat
     9541 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct
     9601 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt
     9661 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg
     9721 gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc
     9781 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg
     9841 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac
     9901 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc
     9961 gggcttgggg gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg
    10021 ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg
    10081 attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca
    10141 tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc
    10201 gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct
    10261 tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg
    10321 gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca
    10381 aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc
    10441 gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg
    10501 cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc
    10561 gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc
    10621 cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta
    10681 agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa
    10741 gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac
    10801 gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac
    10861 ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc
    10921 ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt
    10981 ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat
    11041 cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt
    11101 tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg
    11161 gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc
    11221 tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt
    11281 tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg
    11341 ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct
    11401 cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc
    11461 caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt
    11521 gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg
    11581 cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc
    11641 cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg
    11701 ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc
    11761 gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt
    11821 ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa
    11881 acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt
    11941 cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat
    12001 gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac
    12061 gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt
    12121 tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt
    12181 ggatcaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac
    12241 gaacggataa acct
//
